Human CXCR4 (Chemokine C-X-C-Motif Receptor) ELISA Kit
The Human CXCR4 (Chemokine C-X-C motif receptor 4) ELISA Kit is specifically designed for the precise measurement of CXCR4 levels in human samples, including serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring accurate and consistent results for a variety of research applications.CXCR4 is a key receptor involved in the immune response and plays a critical role in cell migration and survival. Dysregulation of CXCR4 expression has been linked to various diseases, including cancer and inflammatory disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With the Human CXCR4 ELISA Kit, researchers can confidently analyze and quantify CXCR4 levels in human samples, providing valuable insight into the role of this receptor in disease pathology and progression. Trust in the accuracy and reliability of this kit to advance your research efforts in understanding CXCR4 biology and its implications for human health.
Product Name:
Human CXCR4 (Chemokine C-X-C-Motif Receptor) ELISA Kit
SKU:
HUES03471
Target:
Human CXCR4 (Chemokine C-X-C-Motif Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CXCR4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CXCR4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CXCR4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CXCR4. You can calculate the concentration of Human CXCR4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
93-109
92-102
Average (%)
105
101
97
1:4
Range (%)
91-107
86-96
86-100
Average (%)
99
91
91
1:8
Range (%)
86-99
82-93
88-104
Average (%)
93
88
95
1:16
Range (%)
93-104
85-96
82-96
Average (%)
98
91
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
100
EDTA plasma (n=5)
85-101
92
Cell culture media (n=5)
85-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
267.75
731.87
1676.97
256.77
723.66
1746.63
Standard deviation
15.88
32.71
84.18
17.08
39.51
85.76
C V (%)
5.93
4.47
5.02
6.65
5.46
4.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CXCR4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CXCR4 in samples. No significant cross-reactivity or interference between Human CXCR4 and analogues was observed.
