The Human CYB5A (Cytochrome B5) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of cytochrome B5 levels in human samples, including serum, plasma, and cell culture supernatants. This kit provides dependable and reproducible results, making it suitable for a wide range of research applications.Cytochrome B5 is an important protein involved in electron transfer reactions and metabolism, playing a critical role in various cellular processes. Dysregulation of cytochrome B5 has been linked to various health conditions, including metabolic disorders and genetic diseases.
Monitoring cytochrome B5 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.Overall, the Human CYB5A CLIA Kit offers researchers a reliable tool to study the role of cytochrome B5 in health and disease, advancing our understanding of cellular function and enabling the discovery of novel treatment strategies.
Product Name:
Human CYB5A (Cytochrome b5) CLIA Kit
SKU:
HUES00917
Target:
Human CYB5A (Cytochrome b5)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CYB5A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CYB5A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CYB5A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CYB5A. You can calculate the concentration of Human CYB5A in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-111
89-102
86-97
Average (%)
104
96
91
1:4
Range (%)
95-112
94-109
89-100
Average (%)
103
101
95
1:8
Range (%)
103-117
92-107
94-108
Average (%)
108
98
99
1:16
Range (%)
87-101
100-114
94-111
Average (%)
94
106
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-110
104
EDTA plasma (n=5)
88-102
94
Cell culture media (n=5)
93-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.16
179.08
668.16
104.14
177.64
654.12
Standard deviation
12.02
20.88
54.46
13.11
17.05
55.08
C V (%)
12.12
11.66
8.15
12.59
9.6
8.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CYB5A concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CYB5A in samples. No significant cross-reactivity or interference between Human CYB5A and analogues was observed.
