Human CYP1A1 (Cytochrome P450, family 1, subfamily A, polypeptide 1) CLIA Kit
The Human CYP1A1 (Cytochrome P450 Family 1 Subfamily A Polypeptide 1) CLIA Kit is a powerful tool for detecting and quantifying CYP1A1 levels in human samples. This kit is well-suited for use with serum, plasma, and cell culture supernatants, offering high sensitivity and specificity for accurate and reliable results.CYP1A1 is a member of the cytochrome P450 enzyme family, playing a crucial role in drug metabolism and the detoxification of harmful substances in the body. Dysregulation of CYP1A1 has been linked to various diseases, including cancer, cardiovascular disorders, and respiratory diseases, making it a valuable biomarker for research and clinical applications.
With this CLIA kit, researchers can gain insights into CYP1A1 expression levels and activity, paving the way for a better understanding of its role in disease development and progression. This kit is a valuable asset for studying drug metabolism, toxicology, and personalized medicine, offering accurate and reproducible results for a wide range of applications.
Product Name:
Human CYP1A1 (Cytochrome P450, family 1, subfamily A, polypeptide 1) CLIA Kit
SKU:
HUES00260
Target:
Human CYP1A1 (Cytochrome P450, family 1, subfamily A, polypeptide 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CYP1A1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CYP1A1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CYP1A1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CYP1A1. You can calculate the concentration of Human CYP1A1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
95-106
96-109
Average (%)
103
100
101
1:4
Range (%)
92-104
95-108
100-112
Average (%)
99
100
106
1:8
Range (%)
92-106
96-108
98-111
Average (%)
97
102
104
1:16
Range (%)
88-99
95-108
93-106
Average (%)
94
100
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-112
107
EDTA plasma (n=5)
90-108
98
Cell culture media (n=5)
90-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.25
231.78
842.62
119.28
221.63
804.21
Standard deviation
13.72
23.02
70.53
11.1
18.42
69.32
C V (%)
12.44
9.93
8.37
9.31
8.31
8.62
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CYP1A1 concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human CYP1A1 in samples. No significant cross-reactivity or interference between Human CYP1A1 and analogues was observed.
