Human CYP1B1 (Cytochrome P450, family 1, subfamily B, polypeptide 1) CLIA Kit
The Human CYP1B1 (Cytochrome P450 Family 1 Subfamily B Polypeptide 1) CLIA Kit is a reliable and precise tool for detecting CYP1B1 levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides accurate and reproducible results, making it ideal for a variety of research applications in the field of pharmacology and toxicology.CYP1B1 is a key enzyme belonging to the cytochrome P450 family, playing a crucial role in metabolism and detoxification processes in the body. Its dysregulation has been linked to various diseases, including cancer, cardiovascular disorders, and drug metabolism disorders, making it a valuable biomarker for understanding these conditions and developing therapeutic interventions.
Utilizing the Human CYP1B1 CLIA Kit allows researchers to gain insights into the expression levels and activity of CYP1B1, enabling further investigation into its role in disease pathogenesis and drug metabolism. This kit provides a reliable and efficient platform for studying the intricate mechanisms of cytochrome P450 enzymes and their impact on human health.
Product Name:
Human CYP1B1 (Cytochrome P450, family 1, subfamily B, polypeptide 1) CLIA Kit
SKU:
HUES00275
Target:
Human CYP1B1 (Cytochrome P450, family 1, subfamily B, polypeptide 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CYP1B1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CYP1B1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CYP1B1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CYP1B1. You can calculate the concentration of Human CYP1B1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
94-109
102-120
Average (%)
102
101
110
1:4
Range (%)
94-106
104-117
85-99
Average (%)
101
110
92
1:8
Range (%)
101-113
94-107
101-114
Average (%)
107
101
106
1:16
Range (%)
92-107
92-109
100-115
Average (%)
99
99
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
95
EDTA plasma (n=5)
84-99
90
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.19
291.22
905.74
115.25
310.56
945.08
Standard deviation
9.33
28.48
57.33
13.05
36.71
91.86
C V (%)
8.79
9.78
6.33
11.32
11.82
9.72
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CYP1B1 concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human CYP1B1 in samples. No significant cross-reactivity or interference between Human CYP1B1 and analogues was observed.
