The Human CypA (Cyclophilin A) CLIA Kit is designed for the precise measurement of Cyclophilin A levels in human samples including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Cyclophilin A is a key protein known for its role in protein folding and immune response regulation. It is involved in various physiological processes, including viral replication, inflammation, and cell signaling pathways.
Dysregulation of Cyclophilin A has been linked to autoimmune diseases, viral infections, and cardiovascular disorders, highlighting its importance as a potential biomarker for diagnostic and therapeutic development.Overall, the Human CypA CLIA Kit offers researchers a valuable tool for studying the function of Cyclophilin A and its implications in human health and disease.
Product Name:
Human CYPA (Cyclophilin A) CLIA Kit
SKU:
HUES01064
Target:
Human CYPA (Cyclophilin A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CYPA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CYPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CYPA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CYPA. You can calculate the concentration of Human CYPA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
83-96
95-105
Average (%)
101
90
100
1:4
Range (%)
103-115
94-107
94-109
Average (%)
109
102
102
1:8
Range (%)
99-112
95-110
97-110
Average (%)
105
101
104
1:16
Range (%)
93-107
98-109
97-113
Average (%)
100
104
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
101
EDTA plasma (n=5)
94-109
100
Cell culture media (n=5)
89-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.06
3.18
9.95
1.0
3.11
9.14
Standard deviation
0.11
0.25
0.99
0.11
0.36
0.98
C V (%)
10.38
7.86
9.95
11.0
11.58
10.72
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CYPA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CYPA in samples. No significant cross-reactivity or interference between Human CYPA and analogues was observed.
