The Human CYPA (Cyclophilin A) ELISA Kit has been meticulously developed for the precise quantitative analysis of Cyclophilin A in human biological samples. Cyclophilin A is a ubiquitous intracellular protein known for its involvement in protein folding, regulation of immune responses, and diverse cellular functions. Its detection provides valuable insights into cellular processes and potential associations with various physiological and pathological conditions. Our CYPA ELISA Kit boasts exceptional sensitivity and specificity, ensuring the accurate and reproducible measurement of Cyclophilin A levels.
Manufactured under rigorous quality control standards, this kit guarantees robust performance and user-friendly protocols suitable for a range of research and clinical applications. Depend on Assay Genie's CYPA ELISA Kit for the reliable quantification of this essential protein in your studies.
Product Name:
Human CYPA (Cyclophilin A) ELISA Kit
SKU:
AEES00130
Target:
Human CYPA (Cyclophilin A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CYPA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CYPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CYPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CYPA. You can calculate the concentration of Human CYPA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
89-97
86-97
Average (%)
93
93
91
1:4
Range (%)
88-98
97-111
94-103
Average (%)
95
105
100
1:8
Range (%)
94-103
94-106
94-102
Average (%)
100
101
97
1:16
Range (%)
99-111
84-98
95-107
Average (%)
104
92
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
103
EDTA plasma (n=5)
95-103
98
Cell culture media (n=5)
92-100
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
8.29
20.91
90.96
9.58
22.8
97.65
Standard deviation
0.41
0.99
4.7
0.5
1.05
4.9
C V (%)
4.95
4.73
5.17
5.22
4.61
5.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CYPA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CYPA in samples. No significant cross-reactivity or interference between Human CYPA and analogues was observed.
