Human Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61) CLIA Kit
The Human CYR61 (Cysteine-rich protein 61) CLIA Kit is specifically designed for the accurate detection of CYR61 levels in human biological samples such as serum, plasma, and cell culture supernatants. This advanced kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.CYR61, also known as Angiogenic Inducer 61, is a key protein involved in angiogenesis and cell proliferation. It plays a crucial role in various physiological and pathological processes, including cancer, cardiovascular diseases, and neurodegenerative disorders.
By accurately measuring CYR61 levels, researchers can gain valuable insights into these conditions and potentially develop new therapeutic strategies.With its precise detection capabilities, the Human CYR61 CLIA Kit provides a valuable tool for researchers studying angiogenesis and related diseases. Trust in this kit to deliver accurate and dependable results for your research needs.
Product Name:
Human Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61) CLIA Kit
SKU:
HUES00738
Target:
Human Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Cyr61. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Cyr61 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Cyr61, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Cyr61. You can calculate the concentration of Human Cyr61 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-119
92-107
95-108
Average (%)
109
98
103
1:4
Range (%)
97-111
88-98
89-101
Average (%)
105
93
95
1:8
Range (%)
100-118
89-101
97-108
Average (%)
108
94
103
1:16
Range (%)
100-114
85-99
98-112
Average (%)
106
92
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
91-107
99
Cell culture media (n=5)
92-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.48
95.44
402.47
48.18
93.11
393.99
Standard deviation
4.97
7.42
24.27
4.48
7.09
30.97
C V (%)
10.93
7.77
6.03
9.3
7.61
7.86
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Cyr61 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Cyr61 in samples. No significant cross-reactivity or interference between Human Cyr61 and analogues was observed.
