Human Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61) ELISA Kit
The Human CYR61 (Cysteine-rich protein 61) ELISA Kit is specifically designed for the accurate detection of CYR61 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.CYR61 is a key protein known for its role as an angiogenic inducer, promoting angiogenesis and influencing various cellular processes. Its involvement in conditions such as cancer, cardiovascular diseases, and neurodegenerative disorders makes it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.
With the Human CYR61 ELISA Kit, researchers can effectively measure CYR61 levels in biological samples, gaining valuable insights into its role in health and disease. This kit is a valuable tool for advancing research in the field of angiogenesis and related conditions.
Product Name:
Human Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61) ELISA Kit
SKU:
HUES02342
Target:
Human Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Cyr61. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Cyr61 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Cyr61, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Cyr61. You can calculate the concentration of Human Cyr61 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
89-103
92-106
Average (%)
101
95
99
1:4
Range (%)
92-108
82-94
80-94
Average (%)
100
88
87
1:8
Range (%)
92-104
80-94
86-99
Average (%)
98
87
93
1:16
Range (%)
89-102
81-94
88-98
Average (%)
95
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
93
EDTA plasma (n=5)
91-103
98
Cell culture media (n=5)
91-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.37
188.28
957.65
106.56
172.93
978.96
Standard deviation
6.18
11.26
43.0
6.47
9.36
38.77
C V (%)
6.1
5.98
4.49
6.07
5.41
3.96
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Cyr61 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Cyr61 in samples. No significant cross-reactivity or interference between Human Cyr61 and analogues was observed.
