Human CYSLTR1 (Cysteinyl Leukotriene Receptor 1) CLIA Kit
The Human CysLT1R (Cysteinyl Leukotriene Receptor 1) CLIA Kit is specifically designed for the accurate detection of CysLT1R levels in human biological samples such as serum, plasma, and cell culture supernatants. This CLIA kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.CysLT1R is a key receptor involved in the inflammatory response, particularly in conditions such as asthma and allergic rhinitis.
By measuring CysLT1R levels, researchers can gain valuable insights into the role of this receptor in disease pathogenesis and potentially identify new therapeutic targets.Overall, the Human CysLT1R CLIA Kit provides researchers with a reliable tool to study the function and regulation of CysLT1R in various physiological and pathological conditions, ultimately aiding in the development of novel treatment strategies.
Product Name:
Human CYSLTR1 (Cysteinyl Leukotriene Receptor 1) CLIA Kit
SKU:
HUES00589
Target:
Human CYSLTR1 (Cysteinyl Leukotriene Receptor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CYSLTR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CYSLTR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CYSLTR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CYSLTR1. You can calculate the concentration of Human CYSLTR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-117
104-118
90-104
Average (%)
109
110
95
1:4
Range (%)
91-104
91-109
94-109
Average (%)
98
99
100
1:8
Range (%)
100-119
93-109
95-110
Average (%)
108
100
102
1:16
Range (%)
89-101
100-115
100-115
Average (%)
94
108
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-116
106
EDTA plasma (n=5)
99-116
106
Cell culture media (n=5)
99-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
198.99
549.78
1498.68
217.06
604.0
1595.86
Standard deviation
18.07
51.07
121.84
25.77
52.31
110.43
C V (%)
9.08
9.29
8.13
11.87
8.66
6.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CYSLTR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CYSLTR1 in samples. No significant cross-reactivity or interference between Human CYSLTR1 and analogues was observed.
