The Human Cystatin 6 (CST6) ELISA Kit from Assay Genie is a reliable and accurate tool for measuring levels of cystatin 6 in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, providing researchers with dependable and reproducible results for a variety of experimental studies.Cystatin 6 is a vital protein that plays a role in regulating protein degradation and enzyme activity. It is involved in various biological processes, including immune response, inflammation, and tissue remodeling.
Abnormal levels of cystatin 6 have been linked to various diseases, such as cancer, inflammatory disorders, and neurodegenerative conditions.By using the Human Cystatin 6 (CST6) ELISA Kit, researchers can gain valuable insights into the role of cystatin 6 in health and disease. This kit is an essential tool for studying the functions of cystatin 6 and its potential as a biomarker for diagnostic and therapeutic purposes in various medical conditions.
Product Name:
Human Cystatin 6 (CST6) ELISA Kit
Product Code:
HUDL00707
Size:
96 Assays
Target:
CST6
Synonyms:
Cystatin E/M
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
