Human Cysteine Rich Secretory Protein 2 (CRISP2) ELISA Kit
The Human Cysteine-Rich Secretory Protein 2 (CRISP2) ELISA Kit is a reliable tool for the quantitative detection of CRISP2 levels in human samples, including serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures accurate and reproducible results, making it suitable for various research applications.CRISP2 is a protein that plays a crucial role in sperm function and male fertility. It is involved in sperm capacitation, motility, and fertilization, making it an important biomarker for studying male reproductive health and infertility.
Abnormal levels of CRISP2 have been linked to various reproductive disorders, making it a valuable target for therapeutic interventions and diagnostic purposes.Overall, the Human CRISP2 ELISA Kit provides researchers with a powerful tool for investigating the role of CRISP2 in male fertility and reproductive health, offering insights that can contribute to the development of novel treatments and strategies for addressing infertility issues.
Product Name:
Human Cysteine Rich Secretory Protein 2 (CRISP2) ELISA Kit
Product Code:
HUDL00680
Size:
96 Assays
Target:
CRISP2
Synonyms:
TSP1, CRISP-2, GAPDL5, TPX1, CT36, Testis Specific Protein 1, Cancer/Testis Antigen 36, Testis-specific protein TPX-1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
