Human Cysteine-Rich Secretory Protein LCCL Domain-Containing 2 (CRISPLD2) ELISA Kit
The Human CRISPLD2 ELISA Kit offered by Assay Genie is designed for the precise measurement of Cysteine-Rich Secretory Protein LCCL Domain-Containing 2 (CRISPLD2) levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.CRISPLD2 is a protein that plays a crucial role in various biological processes, including immune response regulation and inflammation.
Its dysregulation has been linked to certain diseases and conditions, making it an important biomarker for studying these conditions and potentially developing new therapies.With the Assay Genie Human CRISPLD2 ELISA Kit, researchers can easily and effectively measure CRISPLD2 levels in human samples, leading to valuable insights and advancements in the field of biomedical research.
Product Name:
Human Cysteine-Rich Secretory Protein LCCL Domain-Containing 2 (CRISPLD2) ELISA Kit
Product Code:
HUDL00682
Size:
96 Assays
Target:
CRISPLD2
Synonyms:
Cysteine-rich secretory protein 11(CRISP-11), LCCL domain-containing cysteine-rich secretory protein 2
Detection Method:
Sandwich
Range:
3.12-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
