Human Cysteine Rich With EGF Like Domains Protein 1 (CRELD1) ELISA Kit
The Human Cysteine-Rich with EGF-Like Domains Protein 1 (CRELD1) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of CRELD1 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.CRELD1 is a protein that contains cysteine-rich and EGF-like domains and is involved in various biological processes such as cell adhesion and signaling.
Dysregulation of CRELD1 has been implicated in diseases like cardiovascular disorders and developmental abnormalities, highlighting its importance as a potential biomarker for studying these conditions and developing targeted therapies.With its advanced technology and precise methodology, the CRELD1 ELISA Kit offers researchers a valuable tool for investigating the role of CRELD1 in health and disease, paving the way for new discoveries and therapeutic interventions.
Product Name:
Human Cysteine Rich With EGF Like Domains Protein 1 (CRELD1) ELISA Kit
Product Code:
HUDL00672
Size:
96 Assays
Target:
CRELD1
Synonyms:
AVSD2, CIRRIN, Atrioventricular Septal Defect 2
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
