The Human Cytochrome P450 2C18 (CYP2C18) ELISA Kit is a cutting-edge tool for the precise measurement of CYP2C18 levels in human biological samples. With its advanced technology and high level of accuracy, this kit guarantees consistent and dependable results, making it perfect for a wide variety of research applications.CYP2C18 is a key enzyme involved in drug metabolism and detoxification processes in the body. It plays a crucial role in determining how the body processes various medications, making it a valuable target for studying drug interactions and potential toxicities.
By accurately measuring CYP2C18 levels, researchers can gain valuable insights into personalized medicine, drug development, and pharmacogenomics.Overall, the Human Cytochrome P450 2C18 (CYP2C18) ELISA Kit is an indispensable tool for researchers looking to explore the role of CYP2C18 in drug metabolism, toxicology, and personalized medicine. Its high sensitivity and specificity make it a reliable choice for obtaining precise and reproducible data in their studies.
Product Name:
Human Cytochrome P450 2C18 (CYP2C18) ELISA Kit
Product Code:
HUDL00768
Size:
96 Assays
Target:
CYP2C18
Synonyms:
CYP2C, CPCI, CYP2C17, CPCJ, P450C2C, limonene 6-monooxygenase, Mephenytoin 4-hydroxylase, Cytochrome P450 Family 2 Subfamily C Polypeptide 18
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
