The Human Cytochrome P450 7A1 (CYP7A1) ELISA Kit is a powerful tool designed for the precise measurement of CYP7A1 levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Cytochrome P450 7A1 is a key enzyme involved in the metabolism of cholesterol and bile acid synthesis. Dysregulation of CYP7A1 has been linked to various metabolic disorders, including dyslipidemia and cholestatic liver diseases.
By detecting CYP7A1 levels, researchers can gain insights into the mechanisms underlying these conditions and explore potential therapeutic strategies.With its user-friendly protocol and reliable performance, the Human Cytochrome P450 7A1 (CYP7A1) ELISA Kit is an invaluable tool for studying lipid metabolism, liver function, and related disease pathways. Trust in this kit for accurate and reproducible results that advance your research efforts.
Product Name:
Human Cytochrome P450 7A1 (CYP7A1) ELISA Kit
Product Code:
HUDL00774
Size:
96 Assays
Target:
CYP7A1
Synonyms:
CYP7, CYP7-A1, CYPVII, Cholesterol 7 Alpha Hydroxylase, Cytochrome P450 Family 7 Subfamily A Polypeptide 1
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
