Description
Product Name: | Human Cytomegalovirus (HCMV) IgM ELISA Kit |
SKU: | HDES0028 |
Size: | 96T |
Target: | HCMV-IgM |
Detection Method: | Capture ELISA |
Reactivity: | Human |
Result Type: | Qualitative: Sensitivity 98.9%, Specificity 99.8% |
Sample Type: | Serum, Plasma |
Detection Wavelength: | 450/630nm |
Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Capture-ELISA as the method to detect human Cytomegalo virus IgM antibodies (HCMV-IgM) in serum or plasma samples. The ELISA Microtiter plate provided in this kit has been pre-coated with Mouse-anti-human IgM (μ chain). Samples are added to the ELISA Microtiter plate wells and the IgM antibody in which will be captured. Free components are washed away. Recombinant antigen HCMVrp52-65 conjugated with HRP is added to each well and react with the compound to form “Mouse-anti-human IgM (μ chain)-HCMV IgM - HCMVrp52-65 antigen-HRP” compound. The TMB substrate is added after washing to initiate the color developing reaction. The presence of HCMV-IgM can be determined according to the OD value after colorimetric assay with the Microplate reader.