Description
| Product Name: | Human Cytomegalovirus (HCMV) IgM ELISA Kit |
| SKU: | HDES0028 |
| Size: | 96T |
| Target: | HCMV-IgM |
| Detection Method: | Capture ELISA |
| Reactivity: | Human |
| Result Type: | Qualitative: Sensitivity 98.9%, Specificity 99.8% |
| Sample Type: | Serum, Plasma |
| Detection Wavelength: | 450/630nm |
| Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Capture-ELISA as the method to detect human Cytomegalo virus IgM antibodies (HCMV-IgM) in serum or plasma samples. The ELISA Microtiter plate provided in this kit has been pre-coated with Mouse-anti-human IgM (μ chain). Samples are added to the ELISA Microtiter plate wells and the IgM antibody in which will be captured. Free components are washed away. Recombinant antigen HCMVrp52-65 conjugated with HRP is added to each well and react with the compound to form “Mouse-anti-human IgM (μ chain)-HCMV IgM - HCMVrp52-65 antigen-HRP” compound. The TMB substrate is added after washing to initiate the color developing reaction. The presence of HCMV-IgM can be determined according to the OD value after colorimetric assay with the Microplate reader.