The Human D1R (DRD1) Dopamine Receptor D1 CLIA Kit is designed for the precise measurement of dopamine receptor D1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research applications.The dopamine receptor D1 is a vital component in the central nervous system, playing a key role in regulating behaviors, emotions, and cognitive functions. Dysregulation of this receptor has been linked to various neurological disorders, including schizophrenia, Parkinson's disease, and addiction.
Therefore, the measurement of dopamine receptor D1 levels using this kit can provide valuable insights into the pathophysiology of these conditions and aid in the development of potential treatment strategies.Overall, the Human D1R Dopamine Receptor D1 CLIA Kit is a valuable tool for researchers studying the dopamine system and its implications in neurological disorders, offering reliable and precise measurements for impactful research outcomes.
Product Name:
Human D1R/DRD1 (Dopamine Receptor D1) CLIA Kit
SKU:
HUES00697
Target:
Human D1R/DRD1 (Dopamine Receptor D1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human D1R/DRD1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human D1R/DRD1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human D1R/DRD1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human D1R/DRD1. You can calculate the concentration of Human D1R/DRD1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
91-107
85-97
Average (%)
101
98
91
1:4
Range (%)
102-114
101-118
88-100
Average (%)
108
109
94
1:8
Range (%)
94-111
89-101
96-113
Average (%)
101
95
104
1:16
Range (%)
93-109
100-116
98-112
Average (%)
100
107
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
94-107
102
Cell culture media (n=5)
93-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
199.76
485.03
1849.79
194.12
468.65
1670.02
Standard deviation
17.16
56.41
183.13
15.94
41.76
169.84
C V (%)
8.59
11.63
9.9
8.21
8.91
10.17
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human D1R/DRD1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human D1R/DRD1 in samples. No significant cross-reactivity or interference between Human D1R/DRD1 and analogues was observed.
