The Human DAF (Decay Accelerating Factor) CLIA Kit is a cutting-edge assay designed for the accurate and sensitive detection of DAF levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high specificity and sensitivity, ensuring reliable and reproducible results for a variety of research applications.DAF, also known as CD55, is a key regulator of the complement system and immune response. Dysregulation of DAF expression has been linked to various diseases, including autoimmune disorders, inflammatory conditions, and infectious diseases.
The Human DAF CLIA Kit provides researchers with a valuable tool for studying the role of DAF in disease pathology and developing new therapeutic approaches.With its advanced technology and user-friendly protocol, the Human DAF CLIA Kit from Assay Genie is an essential tool for researchers looking to investigate the role of DAF in health and disease. Order yours today and take your research to the next level.
Product Name:
Human DAF (Decay Accelerating Factor) CLIA Kit
SKU:
HUES01068
Target:
Human DAF (Decay Accelerating Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DAF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DAF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DAF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DAF. You can calculate the concentration of Human DAF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
97-111
85-96
Average (%)
103
103
91
1:4
Range (%)
95-110
87-102
96-110
Average (%)
101
93
104
1:8
Range (%)
94-106
96-107
94-109
Average (%)
99
101
100
1:16
Range (%)
102-117
93-106
92-107
Average (%)
108
101
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
98-116
106
Cell culture media (n=5)
99-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.61
81.96
341.71
38.51
84.34
329.84
Standard deviation
3.26
8.16
38.13
4.19
8.53
30.18
C V (%)
8.44
9.96
11.16
10.88
10.11
9.15
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DAF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DAF in samples. No significant cross-reactivity or interference between Human DAF and analogues was observed.
