The Human DAO (Diamine Oxidase) CLIA Kit is specifically designed for the quantitative detection of diamine oxidase levels in human serum, plasma, and cell culture supernatants. This kit guarantees high sensitivity and accuracy, ensuring precise and consistent results for various research purposes.Diamine oxidase is a key enzyme that plays a vital role in histamine metabolism and regulates immune responses in the body. Dysregulation of DAO levels has been linked to various health conditions, including histamine intolerance, gastrointestinal disorders, and immune system dysfunctions.
Therefore, measuring DAO levels can provide valuable insights into these conditions and aid in the development of targeted treatments.Overall, the Human DAO CLIA Kit offers a reliable and efficient solution for exploring the role of diamine oxidase in health and disease, making it a valuable tool for researchers and healthcare professionals alike.
Product Name:
Human DAO (Diamine Oxidase) CLIA Kit
SKU:
HUES00707
Target:
Human DAO (Diamine Oxidase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DAO. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DAO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DAO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DAO. You can calculate the concentration of Human DAO in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-117
95-106
102-116
Average (%)
108
101
108
1:4
Range (%)
90-101
91-107
85-98
Average (%)
95
98
90
1:8
Range (%)
95-109
103-116
99-113
Average (%)
103
109
105
1:16
Range (%)
94-105
97-112
91-105
Average (%)
100
105
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-111
101
EDTA plasma (n=5)
89-101
96
Cell culture media (n=5)
96-114
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.49
4.7
17.2
2.32
4.23
18.26
Standard deviation
0.23
0.52
1.09
0.26
0.31
1.16
C V (%)
9.24
11.06
6.34
11.21
7.33
6.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DAO concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DAO in samples. No significant cross-reactivity or interference between Human DAO and analogues was observed.
