Human DCLK1 (Doublecortin Like Kinase 1) ELISA Kit
The Human DCLK1 (Doublecortin-like kinase 1) ELISA Kit is a powerful tool for the precise measurement of DCLK1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results that are essential for various research purposes.DCLK1 is a key enzyme involved in regulating cell growth, differentiation, and migration, making it a vital player in many biological processes.
Its dysregulation has been linked to various diseases such as cancer, inflammatory disorders, and neurodegenerative conditions, highlighting its importance as a potential therapeutic target and diagnostic marker.By utilizing the Human DCLK1 ELISA Kit, researchers can gain valuable insights into the role of DCLK1 in disease progression and development, paving the way for innovative treatment strategies and personalized medicine approaches.
Product Name:
Human DCLK1 (Doublecortin Like Kinase 1) ELISA Kit
SKU:
HUES01585
Target:
Human DCLK1 (Doublecortin Like Kinase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DCLK1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DCLK1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DCLK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DCLK1. You can calculate the concentration of Human DCLK1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
99-113
99-116
Average (%)
94
106
106
1:4
Range (%)
91-104
86-101
86-100
Average (%)
98
92
91
1:8
Range (%)
90-102
87-98
83-97
Average (%)
95
92
90
1:16
Range (%)
92-108
85-95
83-93
Average (%)
99
90
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
93
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.17
4.32
0.54
1.17
4.45
Standard deviation
0.03
0.06
0.17
0.03
0.06
0.17
C V (%)
5.45
5.13
3.94
5.56
5.13
3.82
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human DCLK1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DCLK1 in samples. No significant cross-reactivity or interference between Human DCLK1 and analogues was observed.
