The Human DCN (Decorin) CLIA Kit is a high-quality assay designed for the precise measurement of decorin levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Decorin is a vital extracellular matrix protein that plays a critical role in regulating collagen fibrillogenesis and cell growth. It is involved in various biological processes, including tissue remodeling, wound healing, and inflammation.
Abnormal decorin levels have been associated with diseases such as cancer, fibrosis, and arthritis, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.The Human DCN (Decorin) CLIA Kit offers a reliable and easy-to-use solution for researchers looking to investigate the role of decorin in health and disease. Its robust performance and user-friendly design make it an excellent choice for laboratories seeking accurate and reproducible data in their studies.
Product Name:
Human DCN (Decorin) CLIA Kit
SKU:
HUES01179
Target:
Human DCN (Decorin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DCN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DCN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DCN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DCN. You can calculate the concentration of Human DCN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
102-116
99-111
Average (%)
93
108
105
1:4
Range (%)
89-103
104-115
99-111
Average (%)
94
109
105
1:8
Range (%)
87-102
94-109
91-101
Average (%)
93
100
96
1:16
Range (%)
94-106
93-109
91-103
Average (%)
100
101
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-117
107
EDTA plasma (n=5)
94-110
102
Cell culture media (n=5)
96-107
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.98
2.76
8.16
1.06
2.83
8.15
Standard deviation
0.08
0.23
0.75
0.13
0.26
0.74
C V (%)
8.16
8.33
9.19
12.26
9.19
9.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DCN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DCN in samples. No significant cross-reactivity or interference between Human DCN and analogues was observed.
