Human DEF alpha1 (Defensin Alpha 1, Neutrophil) CLIA Kit
The Human Def Alpha1 Defensin Alpha 1 (Neutrophil) CLIA Kit is a cutting-edge assay designed for the precise quantification of Def Alpha1 Defensin Alpha 1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Def Alpha1 Defensin Alpha 1, a key component of the immune system, plays a vital role in defending the body against microbial infections.
Its dysregulation has been implicated in various inflammatory conditions, autoimmune diseases, and infections, underscoring its importance as a potential biomarker for disease prognosis and therapeutic development.With this advanced CLIA kit, researchers can gain valuable insights into the role of Def Alpha1 Defensin Alpha 1 in various physiological and pathological conditions, paving the way for innovative diagnostic and therapeutic strategies.
Product Name:
Human DEF alpha1 (Defensin Alpha 1, Neutrophil) CLIA Kit
SKU:
HUES00510
Target:
Human DEF alpha1 (Defensin Alpha 1, Neutrophil)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DEFα1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DEFα1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DEFα1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DEFα1. You can calculate the concentration of Human DEFα1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-120
93-106
86-100
Average (%)
109
100
93
1:4
Range (%)
97-111
88-100
101-117
Average (%)
105
93
109
1:8
Range (%)
91-103
87-102
102-118
Average (%)
98
94
108
1:16
Range (%)
90-105
91-103
96-110
Average (%)
97
97
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-115
107
EDTA plasma (n=5)
96-110
103
Cell culture media (n=5)
88-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.07
116.63
466.5
47.3
112.4
471.82
Standard deviation
5.45
14.0
44.6
4.9
12.59
37.42
C V (%)
11.58
12.0
9.56
10.36
11.2
7.93
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DEFα1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DEFα1 in samples. No significant cross-reactivity or interference between Human DEFα1 and analogues was observed.
