The Human DEFB1 (Defensin Beta 1) CLIA Kit is a powerful tool for the quantitative measurement of DEFB1 levels in human samples including serum, plasma, and cell culture supernatants. This innovative kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.DEFB1 is an important antimicrobial peptide that plays a crucial role in the innate immune response, helping to protect against bacterial, viral, and fungal infections. Dysregulation of DEFB1 expression has been associated with various diseases, making it a valuable biomarker for studying immune-related disorders and potential therapeutic interventions.
With the Human DEFB1 CLIA Kit, researchers can gain valuable insights into the role of DEFB1 in health and disease, providing a deeper understanding of the immune system and its impact on human health. This kit is a reliable and versatile tool for researchers looking to investigate the function of DEFB1 and its potential as a target for therapeutic interventions.
Product Name:
Human DEFB1 (Defensin Beta 1) CLIA Kit
SKU:
HUES00587
Target:
Human DEFB1 (Defensin Beta 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DEFB1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DEFB1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DEFB1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DEFB1. You can calculate the concentration of Human DEFB1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-96
100-112
89-102
Average (%)
90
106
96
1:4
Range (%)
91-103
97-115
96-111
Average (%)
96
105
104
1:8
Range (%)
95-108
90-100
94-105
Average (%)
101
95
99
1:16
Range (%)
88-102
90-103
90-103
Average (%)
95
96
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
99
EDTA plasma (n=5)
91-105
98
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.48
150.66
477.74
46.71
140.15
469.68
Standard deviation
5.14
13.73
42.09
5.06
10.75
43.12
C V (%)
10.18
9.11
8.81
10.83
7.67
9.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DEFB1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DEFB1 in samples. No significant cross-reactivity or interference between Human DEFB1 and analogues was observed.
