The Human DEFB1 (Defensin Beta 1) ELISA Kit is specifically designed for the precise quantification of defensin beta 1 levels in human serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for various research applications.Defensin beta 1 is a key component of the innate immune system, known for its antimicrobial properties and ability to combat pathogens. Research has shown its involvement in inflammatory processes, wound healing, and defense against infections, highlighting its significance as a biomarker in understanding immune responses and diseases.
By utilizing the Human DEFB1 ELISA Kit, researchers can gain valuable insights into the role of defensin beta 1 in various physiological and pathological conditions, paving the way for potential therapeutic interventions and advancements in the field of immunoassay research.
Product Name:
Human DEFB1 (Defensin Beta 1) ELISA Kit
SKU:
HUES02107
Target:
Human DEFB1 (Defensin Beta 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DEFB1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DEFB1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DEFB1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DEFB1. You can calculate the concentration of Human DEFB1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
91-104
97-110
Average (%)
98
98
104
1:4
Range (%)
93-104
85-98
85-97
Average (%)
98
91
90
1:8
Range (%)
87-100
80-94
82-94
Average (%)
93
86
89
1:16
Range (%)
90-103
82-95
86-101
Average (%)
98
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
96
EDTA plasma (n=5)
87-97
92
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.42
291.69
820.81
101.98
320.07
801.87
Standard deviation
5.2
17.03
41.45
6.37
13.96
38.09
C V (%)
5.45
5.84
5.05
6.25
4.36
4.75
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human DEFB1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DEFB1 in samples. No significant cross-reactivity or interference between Human DEFB1 and analogues was observed.
