The Human Deoxyribonuclease I (DNase1) ELISA Kit is a cutting-edge tool for the precise measurement of DNase1 levels in human biological samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it a valuable resource for a variety of research endeavors.DNase1 is a key enzyme involved in the breakdown of DNA, playing a critical role in various physiological processes including cell death, immune response, and tissue repair. Dysregulation of DNase1 activity has been linked to autoimmune diseases, inflammatory conditions, and cardiovascular disorders, highlighting its significance as a potential biomarker for studying these pathologies and developing novel therapeutic strategies.
By utilizing the Human Deoxyribonuclease I (DNase1) ELISA Kit, researchers can gain valuable insights into the role of DNase1 in health and disease, paving the way for advancements in diagnostics and treatment options. Trust in this innovative kit to drive your research forward with precision and reliability.
Product Name:
Human Deoxyribonuclease I (DNASE1) ELISA Kit
Product Code:
HUDL00849
Size:
96 Assays
Target:
DNASE1
Synonyms:
DNaseI, DNL1, DRNI, DNase-I, Dornase alfa
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
