The Human DES (Desmin) CLIA Kit is a cutting-edge diagnostic tool specifically designed for the precise measurement of desmin levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Desmin is a vital structural protein found in muscle tissues, playing a crucial role in maintaining cell shape and function.
Aberrant desmin levels have been linked to various muscle disorders, including muscular dystrophy and cardiomyopathy, making it a key biomarker for understanding these conditions and developing effective treatments.With the Human DES (Desmin) CLIA Kit, researchers and clinicians can now explore the intricate mechanisms underlying muscle diseases with precision and reliability, ultimately advancing our knowledge and capabilities in the field of medicine.
Product Name:
Human Des (Desmin) CLIA Kit
SKU:
HUES00854
Target:
Human Des (Desmin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Des. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Des and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Des, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Des. You can calculate the concentration of Human Des in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
101-116
90-106
Average (%)
94
107
98
1:4
Range (%)
90-100
92-104
98-113
Average (%)
95
99
105
1:8
Range (%)
93-107
103-118
100-116
Average (%)
99
109
106
1:16
Range (%)
96-109
90-102
100-117
Average (%)
102
96
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-112
106
EDTA plasma (n=5)
96-111
103
Cell culture media (n=5)
98-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.99
195.46
892.48
113.25
214.28
975.46
Standard deviation
12.48
19.72
86.84
14.12
16.07
104.37
C V (%)
11.89
10.09
9.73
12.47
7.5
10.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Des concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Des in samples. No significant cross-reactivity or interference between Human Des and analogues was observed.
