Human DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit
The Human DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit is specially designed for the precise measurement of DHEA-S levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for various research applications.DHEA-S is a key steroid hormone that serves as a precursor to other hormones in the body, playing a vital role in numerous physiological processes.
Abnormal levels of DHEA-S have been associated with various health conditions, including hormonal imbalances, autoimmune disorders, and aging-related issues. As such, this ELISA kit provides researchers with a valuable tool for studying these conditions and exploring potential therapeutic interventions.
Product Name:
Human DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit
SKU:
HUES02948
Target:
Human DHEA-S (Dehydroepiandrosterone Sulfate)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DHEA-S. During the reaction, DHEA-S in the sample or standard competes with a fixed amount of DHEA-S on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DHEA-S. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DHEA-S in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
87-98
99-112
Average (%)
91
92
104
1:4
Range (%)
82-94
92-104
98-115
Average (%)
89
98
106
1:8
Range (%)
87-101
89-103
96-111
Average (%)
93
94
103
1:16
Range (%)
84-95
93-109
96-112
Average (%)
90
99
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
88-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.3
89.6
442.4
44.5
84.1
469.9
Standard deviation
3.2
4.7
14.6
2.4
5.0
22.6
C V (%)
6.91
5.25
3.3
5.39
5.95
4.81
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of DHEA-S concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes DHEA-S in samples. No significant cross-reactivity or interference between DHEA-S and analogues was observed.
