Human Dicer 1, Ribonuclease Type III (DICER1) ELISA Kit
The Human DICER-1 (Ribonuclease Type III) ELISA Kit is specifically designed for the precise detection of DICER-1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and reproducible results for a variety of research purposes.DICER-1 is a key enzyme involved in RNA interference, playing a crucial role in the regulation of gene expression and the processing of microRNAs. Dysregulation of DICER-1 has been linked to various diseases, including cancer, autoimmune disorders, and viral infections, highlighting its significance as a potential diagnostic and therapeutic target in medical research.
By accurately measuring DICER-1 levels in biological samples, researchers can gain valuable insights into the underlying mechanisms of disease pathology and identify novel strategies for treatment and intervention. The Human DICER-1 ELISA Kit provides a valuable tool for studying the intricate functions of this essential enzyme and its implications in human health and disease.
Product Name:
Human Dicer 1, Ribonuclease Type III (DICER1) ELISA Kit
Product Code:
HUDL00829
Size:
96 Assays
Target:
DICER1
Synonyms:
Dicer, HERNA, Dicer 1,Double-Stranded RNA-Specific Endoribonuclease, Helicase with RNase motif, Helicase MOI
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
