The Human DLK-1 (Delta-like 1 homolog) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of DLK-1 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for various research applications.DLK-1 is a critical protein involved in cell signaling and development, playing a key role in processes such as differentiation, proliferation, and apoptosis.
Dysregulation of DLK-1 has been implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human DLK-1 CLIA Kit offers researchers a precise and efficient method for quantifying DLK-1 levels, facilitating deeper insights into the role of this protein in health and disease.
Product Name:
Human DLK-1 (Delta Like 1 Homolog) CLIA Kit
SKU:
HUES00208
Target:
Human DLK-1 (Delta Like 1 Homolog)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DLK-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DLK-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DLK-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DLK-1. You can calculate the concentration of Human DLK-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
85-97
91-105
Average (%)
107
90
97
1:4
Range (%)
90-106
99-109
103-120
Average (%)
97
104
110
1:8
Range (%)
91-106
87-101
98-112
Average (%)
99
93
104
1:16
Range (%)
87-102
97-115
85-98
Average (%)
94
105
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
90
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.75
200.79
694.27
96.69
204.04
671.36
Standard deviation
9.52
22.95
65.89
8.67
22.79
53.71
C V (%)
9.94
11.43
9.49
8.97
11.17
8.0
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DLK-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DLK-1 in samples. No significant cross-reactivity or interference between Human DLK-1 and analogues was observed.
