The Human DMD (Dystrophin) CLIA Kit is a cutting-edge diagnostic tool specifically designed for the precise measurement of Dystrophin levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers unmatched sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Dystrophin is a critical protein responsible for maintaining the structural integrity of muscle fibers. Mutations in the DMD gene can lead to Duchenne muscular dystrophy (DMD), a debilitating muscle-wasting disease.
The ability to accurately measure Dystrophin levels is essential for understanding the progression of DMD and developing potential therapeutic interventions.By using the Human DMD CLIA Kit, researchers can gain valuable insights into the role of Dystrophin in muscle health and disease pathology. This kit is a valuable tool for advancing research in the field of muscular dystrophy and holds great promise for improving diagnostic and treatment strategies for patients affected by DMD.
Product Name:
Human DMD (Dystrophin) CLIA Kit
SKU:
HUES00814
Target:
Human DMD (Dystrophin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DMD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DMD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DMD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DMD. You can calculate the concentration of Human DMD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
87-98
99-114
Average (%)
102
92
104
1:4
Range (%)
95-108
86-98
90-105
Average (%)
101
92
96
1:8
Range (%)
97-109
97-110
102-115
Average (%)
103
104
108
1:16
Range (%)
95-109
100-111
94-110
Average (%)
102
106
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-113
105
EDTA plasma (n=5)
100-114
108
Cell culture media (n=5)
90-106
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.54
224.07
923.09
105.32
227.03
876.23
Standard deviation
11.04
23.17
92.03
9.26
21.61
95.42
C V (%)
10.56
10.34
9.97
8.79
9.52
10.89
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DMD concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DMD in samples. No significant cross-reactivity or interference between Human DMD and analogues was observed.
