The Human DNase I (Deoxyribonuclease I) ELISA Kit is specifically designed for the precise measurement of DNase I levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and selectivity to ensure accurate and consistent results, making it an invaluable tool for various research applications.DNase I is an essential enzyme responsible for breaking down DNA into smaller fragments, playing a crucial role in various biological processes such as DNA repair, cell death, and immune response.
Dysregulation of DNase I has been linked to autoimmune diseases, cancer, and inflammatory conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.Overall, the Human DNase I ELISA Kit provides researchers with a reliable and efficient method for quantifying DNase I levels, enabling a deeper understanding of its role in health and disease.
Product Name:
Human DNase-I (Deoxyribonuclease I) ELISA Kit
SKU:
HUES02950
Target:
Human DNase-I (Deoxyribonuclease I)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DNase-â… . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DNase-â… and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DNase-â… , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DNase-â… . You can calculate the concentration of Human DNase-â… in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
91-103
86-99
Average (%)
105
96
93
1:4
Range (%)
90-105
84-99
88-100
Average (%)
98
91
93
1:8
Range (%)
91-102
87-101
86-99
Average (%)
96
93
93
1:16
Range (%)
89-103
85-97
83-94
Average (%)
94
90
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.21
3.89
0.52
1.24
3.97
Standard deviation
0.04
0.07
0.15
0.03
0.06
0.16
C V (%)
7.55
5.79
3.86
5.77
4.84
4.03
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human DNase-â… concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DNase-â… in samples. No significant cross-reactivity or interference between Human DNase-â… and analogues was observed.
