The Human DNM1 (Dynamin 1) CLIA Kit is specifically designed for the quantitative measurement of DNM1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Dynamin 1 is a key protein involved in the process of endocytosis, playing a critical role in membrane trafficking and vesicle formation.
Dysregulation of DNM1 has been implicated in various diseases including neurodegenerative disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its user-friendly format and robust performance, the Human DNM1 CLIA Kit is a valuable tool for researchers seeking to investigate the role of dynamin 1 in health and disease.
Product Name:
Human DNM1 (Dynamin 1) CLIA Kit
SKU:
HUES00710
Target:
Human DNM1 (Dynamin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DNM1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DNM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DNM1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DNM1. You can calculate the concentration of Human DNM1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-113
95-109
96-109
Average (%)
103
103
103
1:4
Range (%)
87-99
87-99
97-115
Average (%)
94
93
105
1:8
Range (%)
90-106
85-100
89-103
Average (%)
97
92
94
1:16
Range (%)
101-114
94-108
102-114
Average (%)
107
99
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-96
90
EDTA plasma (n=5)
97-113
104
Cell culture media (n=5)
100-113
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
233.59
449.68
2231.5
214.88
427.54
2234.09
Standard deviation
22.7
51.08
254.61
26.09
40.83
232.12
C V (%)
9.72
11.36
11.41
12.14
9.55
10.39
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DNM1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DNM1 in samples. No significant cross-reactivity or interference between Human DNM1 and analogues was observed.
