The Human DNM2 (Dynamin 2) CLIA Kit is specifically designed for the quantitative measurement of DNM2 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reliable results, making it an invaluable tool for various research applications.Dynamin 2 is a key protein involved in endocytosis and membrane trafficking processes in cells. Dysregulation of DNM2 has been linked to various diseases, including Charcot-Marie-Tooth disease, centronuclear myopathy, and certain cancers.
Therefore, measuring DNM2 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Human DNM2 CLIA Kit offers researchers a comprehensive solution for studying the role of Dynamin 2 in health and disease, aiding in the advancement of biomedical research and drug development efforts.
Product Name:
Human DNM2 (Dynamin 2) CLIA Kit
SKU:
HUES00711
Target:
Human DNM2 (Dynamin 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DNM2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DNM2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DNM2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DNM2. You can calculate the concentration of Human DNM2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
99-114
94-108
Average (%)
99
106
101
1:4
Range (%)
100-115
96-112
88-102
Average (%)
107
104
93
1:8
Range (%)
87-102
95-108
96-111
Average (%)
95
103
102
1:16
Range (%)
104-119
98-114
92-104
Average (%)
110
106
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-115
106
EDTA plasma (n=5)
88-100
93
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
265.72
679.15
2300.15
282.12
708.89
2278.93
Standard deviation
32.1
51.0
194.59
34.9
61.67
253.42
C V (%)
12.08
7.51
8.46
12.37
8.7
11.12
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DNM2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DNM2 in samples. No significant cross-reactivity or interference between Human DNM2 and analogues was observed.
