Human Doublecortin Like Kinase 1 (DCLK1) ELISA Kit
The Human Doublecortin-Like Kinase 1 (DCLK1) ELISA Kit is designed for the accurate detection of DCLK1 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring trustworthy and reproducible results that are perfect for a variety of research applications.DCLK1 is a protein that plays a critical role in various cellular processes, including cell proliferation, differentiation, and migration.
It is involved in tumorigenesis, making it a valuable biomarker for studying cancer and potentially developing targeted therapies.With this ELISA kit, researchers can confidently measure DCLK1 levels in biological samples, giving them valuable insights into its role in disease processes and potential therapeutic interventions. Order your Human DCLK1 ELISA Kit today and take your research to the next level.
Product Name:
Human Doublecortin Like Kinase 1 (DCLK1) ELISA Kit
Product Code:
HUDL00797
Size:
96 Assays
Target:
DCLK1
Synonyms:
DCDC3A, DCLK, DCAMKL1, Doublecortin And CaM Kinase-Like 1, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
