The Human DPT (Dermatopontin) CLIA Kit is specifically designed for the accurate detection of DPT levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it a valuable tool for various research applications.Dermatopontin, also known as DPT, is a critical protein involved in skin development and wound healing processes. It plays a crucial role in maintaining skin integrity and elasticity, making it a key biomarker for studying skin disorders, aging, and tissue regeneration.
By accurately measuring DPT levels, researchers can gain insights into potential therapeutic strategies for skin-related conditions.Overall, the Human DPT CLIA Kit offers researchers a reliable and efficient method for quantifying DPT levels in human samples, providing valuable information for advancing research in dermatology, wound healing, and regenerative medicine.
Product Name:
Human DPT (Dermatopontin) CLIA Kit
SKU:
HUES00999
Target:
Human DPT (Dermatopontin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DPT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DPT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DPT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DPT. You can calculate the concentration of Human DPT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-102
90-107
92-107
Average (%)
93
97
98
1:4
Range (%)
93-108
101-113
101-117
Average (%)
100
106
109
1:8
Range (%)
86-99
85-96
92-103
Average (%)
91
90
97
1:16
Range (%)
92-108
102-115
94-108
Average (%)
100
108
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-115
106
EDTA plasma (n=5)
85-98
90
Cell culture media (n=5)
96-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.44
188.75
936.59
94.18
195.63
1021.27
Standard deviation
9.65
20.59
94.97
11.89
22.87
92.83
C V (%)
9.8
10.91
10.14
12.62
11.69
9.09
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DPT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DPT in samples. No significant cross-reactivity or interference between Human DPT and analogues was observed.
