The Human DSP (Desmoplakin) CLIA Kit is specifically designed for the quantitative measurement of desmoplakin levels in human biological samples, such as serum and plasma. This kit offers high precision and sensitivity, allowing for accurate and reliable results for research purposes.Desmoplakin is a key protein essential for maintaining the structural integrity of desmosomes in epithelial cells, contributing to cell-cell adhesion and tissue stability.
Dysregulation of desmoplakin has been linked to various skin disorders, including pemphigus vulgaris and arrhythmogenic right ventricular cardiomyopathy.By utilizing the Human DSP CLIA Kit, researchers can gain valuable insights into the role of desmoplakin in disease pathogenesis and potentially identify novel therapeutic targets for the treatment of desmosome-related disorders.
Product Name:
Human DSP (Desmoplakin) CLIA Kit
SKU:
HUES01023
Target:
Human DSP (Desmoplakin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DSP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DSP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DSP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DSP. You can calculate the concentration of Human DSP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
94-111
93-108
Average (%)
94
101
99
1:4
Range (%)
101-116
98-113
85-97
Average (%)
107
104
90
1:8
Range (%)
96-113
90-104
96-114
Average (%)
103
97
104
1:16
Range (%)
92-107
96-110
92-110
Average (%)
99
103
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-104
98
EDTA plasma (n=5)
93-107
101
Cell culture media (n=5)
98-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
209.51
451.06
1463.48
203.95
469.6
1495.1
Standard deviation
25.14
40.78
140.35
19.8
42.5
98.68
C V (%)
12.0
9.04
9.59
9.71
9.05
6.6
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DSP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DSP in samples. No significant cross-reactivity or interference between Human DSP and analogues was observed.
