The Human Dysferlin (DYSF) ELISA Kit is specifically designed for the precise measurement of dysferlin levels in human samples, including serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Dysferlin is a key protein involved in muscle membrane repair, making it essential for maintaining muscle integrity and function. Dysferlin deficiencies are linked to muscular dystrophies, such as limb-girdle muscular dystrophy and Miyoshi myopathy.
By studying dysferlin levels, researchers can gain valuable insights into muscle-related disorders and potentially develop targeted therapies for these conditions.This ELISA kit provides researchers with a powerful tool for investigating dysferlin biology and its implications in muscle health and disease. It offers a reliable and efficient method for quantifying dysferlin levels, ultimately advancing our understanding of muscle pathology and treatment strategies.
Product Name:
Human Dysferlin (DYSF) ELISA Kit
Product Code:
HUDL00895
Size:
96 Assays
Target:
DYSF
Synonyms:
FER1L1, LGMD2B, Dysferlin,Limb Girdle Muscular Dystrophy 2B(Autosomal Recessive), Fer-1-like protein 1, Dystrophy-associated fer-1-like protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
