Human ECM1 (Extracellular Matrix Protein 1) CLIA Kit
The Human ECM1 (Extracellular Matrix Protein 1) CLIA Kit is a reliable tool for the quantitative detection of ECM1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.ECM1 is an important protein involved in the regulation of extracellular matrix formation, cell adhesion, and tissue development.
Dysregulation of ECM1 is associated with various diseases, including cancer, fibrosis, and inflammatory disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human ECM1 CLIA Kit is a valuable resource for researchers looking to investigate the role of ECM1 in physiological and pathological processes, providing precise and reliable data for their studies.
Product Name:
Human ECM1 (Extracellular Matrix Protein 1) CLIA Kit
SKU:
HUES01110
Target:
Human ECM1 (Extracellular Matrix Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ECM1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ECM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ECM1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ECM1. You can calculate the concentration of Human ECM1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
103-116
96-112
Average (%)
98
109
104
1:4
Range (%)
86-99
90-107
95-108
Average (%)
92
98
100
1:8
Range (%)
90-106
91-106
99-114
Average (%)
98
99
107
1:16
Range (%)
95-110
102-117
90-103
Average (%)
103
108
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
94
EDTA plasma (n=5)
96-110
103
Cell culture media (n=5)
89-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.02
79.36
381.87
44.08
80.36
385.09
Standard deviation
4.88
7.56
33.64
4.82
7.88
33.43
C V (%)
11.9
9.53
8.81
10.93
9.81
8.68
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ECM1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ECM1 in samples. No significant cross-reactivity or interference between Human ECM1 and analogues was observed.
