Human Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2) ELISA Kit
The Human Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2) ELISA Kit is a cutting-edge tool for the precise measurement of ENPP2 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a wide range of research applications.ENPP2, also known as Autotaxin, is a key enzyme involved in the production of lysophosphatidic acid (LPA), which plays a critical role in various cellular processes including cell migration, survival, and proliferation.
Dysregulation of ENPP2/LPA signaling has been implicated in cancer, inflammation, fibrosis, and other diseases, making it a valuable biomarker for studying these conditions and developing targeted therapies.By using the Human ENPP2 ELISA Kit, researchers can gain valuable insights into the role of ENPP2 in health and disease, paving the way for new diagnostic and therapeutic approaches. Trust in the reliability and precision of this ELISA kit to advance your research and uncover the secrets of ENPP2 biology.
Product Name:
Human Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2) ELISA Kit
Product Code:
HUDL00950
Size:
96 Assays
Target:
ENPP2
Synonyms:
ATX, ATX-X, LysoPLD, NPP2, PD-IALPHA, PDNP2, Autotaxin, Extracellular lysophospholipase D
Detection Method:
Sandwich
Range:
93.75-6000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
