The Human EFNA1 (Ephrin-A1) CLIA Kit is a cutting-edge assay designed for the precise measurement of EFNA1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.EFNA1, also known as Ephrin-A1, is a critical protein involved in cell-cell communication and signaling processes. It plays a key role in various physiological and pathological processes, including neural development, inflammation, and cancer progression.
By measuring EFNA1 levels, researchers can gain valuable insights into the underlying mechanisms of these conditions and potentially identify novel therapeutic targets.Overall, the Human EFNA1 CLIA Kit is an indispensable tool for researchers seeking to explore the role of EFNA1 in health and disease, enabling groundbreaking discoveries and advancements in the field of biomedical research.
Product Name:
Human EFNA1 (Ephrin A1) CLIA Kit
SKU:
HUES00528
Target:
Human EFNA1 (Ephrin A1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EFNA1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EFNA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EFNA1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EFNA1. You can calculate the concentration of Human EFNA1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
94-108
87-100
Average (%)
108
100
93
1:4
Range (%)
90-105
101-114
93-105
Average (%)
96
107
99
1:8
Range (%)
102-115
85-99
102-116
Average (%)
108
92
110
1:16
Range (%)
86-99
87-99
86-98
Average (%)
92
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
94
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
90-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.41
272.79
849.06
96.49
267.35
772.86
Standard deviation
8.77
22.31
55.27
8.8
30.26
54.56
C V (%)
8.73
8.18
6.51
9.12
11.32
7.06
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EFNA1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EFNA1 in samples. No significant cross-reactivity or interference between Human EFNA1 and analogues was observed.
