The Human EFNA4 (Ephrin-A4) CLIA Kit is a cutting-edge assay designed for the precise measurement of Ephrin-A4 levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for various research applications.Ephrin-A4 is a key protein involved in cell signaling and tissue development, playing a crucial role in processes such as axon guidance, synaptic plasticity, and immune response regulation.
Dysregulation of Ephrin-A4 has been linked to various diseases including cancer, neurodevelopmental disorders, and autoimmune conditions, making it a promising biomarker for understanding disease mechanisms and exploring potential therapeutic interventions.Trust the Human EFNA4 CLIA Kit from Assay Genie to provide reliable data and support your research efforts in unraveling the complexities of Ephrin-A4 biology and its implications in health and disease.
Product Name:
Human EFNA4 (Ephrin A4) CLIA Kit
SKU:
HUES00650
Target:
Human EFNA4 (Ephrin A4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EFNA4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EFNA4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EFNA4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EFNA4. You can calculate the concentration of Human EFNA4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
96-109
84-99
Average (%)
107
102
91
1:4
Range (%)
94-111
100-118
87-101
Average (%)
102
109
94
1:8
Range (%)
104-120
99-114
86-98
Average (%)
110
106
93
1:16
Range (%)
92-105
96-109
101-114
Average (%)
100
102
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
92
EDTA plasma (n=5)
99-115
106
Cell culture media (n=5)
99-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
204.15
467.51
1666.97
215.83
494.87
1636.15
Standard deviation
18.88
40.72
173.36
26.16
41.42
119.11
C V (%)
9.25
8.71
10.4
12.12
8.37
7.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EFNA4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EFNA4 in samples. No significant cross-reactivity or interference between Human EFNA4 and analogues was observed.
