The Human EFNA5 (Ephrin A5) CLIA Kit is a cutting-edge assay designed for the precise measurement of EFNA5 levels in human samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for various research applications.EFNA5, also known as Ephrin A5, is a critical protein involved in neural development and synaptic plasticity.
Its dysregulation has been linked to neurological disorders such as Alzheimer's disease and schizophrenia, highlighting its importance as a potential biomarker for studying and treating these conditions.With the Human EFNA5 CLIA Kit, researchers can explore the role of EFNA5 in various physiological and pathological processes, paving the way for new insights and therapeutic interventions in neuroscience and beyond.
Product Name:
Human EFNA5 (Ephrin A5) CLIA Kit
SKU:
HUES00663
Target:
Human EFNA5 (Ephrin A5)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EFNA5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EFNA5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EFNA5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EFNA5. You can calculate the concentration of Human EFNA5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
87-100
88-100
Average (%)
96
93
93
1:4
Range (%)
98-114
94-108
97-114
Average (%)
104
102
105
1:8
Range (%)
104-119
90-106
94-107
Average (%)
110
98
100
1:16
Range (%)
97-109
92-108
86-100
Average (%)
103
99
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
108
EDTA plasma (n=5)
88-100
95
Cell culture media (n=5)
86-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.75
308.4
967.24
94.66
332.85
951.96
Standard deviation
8.99
28.87
61.9
8.07
31.19
85.2
C V (%)
8.58
9.36
6.4
8.53
9.37
8.95
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EFNA5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EFNA5 in samples. No significant cross-reactivity or interference between Human EFNA5 and analogues was observed.
