Human EGR1 (Early Growth Response Protein 1) CLIA Kit
The Human EGR1 (Early Growth Response Protein 1) CLIA Kit is a reliable and accurate tool for the quantitative measurement of EGR1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.EGR1 is a key transcription factor that regulates the expression of genes involved in cell growth, differentiation, and response to various stimuli.
Dysregulation of EGR1 has been linked to various diseases, including cancer, inflammatory disorders, and cardiovascular diseases, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.With its advanced technology and user-friendly protocols, the Human EGR1 CLIA Kit from Assay Genie is an essential tool for researchers and clinicians looking to study the role of EGR1 in health and disease.
Product Name:
Human EGR1 (Early Growth Response Protein 1) CLIA Kit
SKU:
HUES01185
Target:
Human EGR1 (Early Growth Response Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EGR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EGR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EGR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EGR1. You can calculate the concentration of Human EGR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
92-104
99-114
Average (%)
93
99
105
1:4
Range (%)
95-107
88-98
97-114
Average (%)
102
93
105
1:8
Range (%)
97-109
101-116
92-106
Average (%)
103
108
99
1:16
Range (%)
97-112
101-114
97-112
Average (%)
102
107
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
106
EDTA plasma (n=5)
89-102
94
Cell culture media (n=5)
86-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.01
174.56
894.64
85.87
171.39
905.98
Standard deviation
10.16
15.1
81.05
7.62
16.66
84.62
C V (%)
10.81
8.65
9.06
8.87
9.72
9.34
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EGR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EGR1 in samples. No significant cross-reactivity or interference between Human EGR1 and analogues was observed.
