The Human Elastase-2B (ELA2B) ELISA Kit is specifically designed for the accurate and precise measurement of elastase-2B levels in human serum, plasma, and cell culture supernatants. Elastase-2B is an important enzyme that plays a key role in the immune response and inflammation processes in the body.This ELISA kit offers high sensitivity and specificity, ensuring reliable and reproducible results for your research needs. By accurately measuring elastase-2B levels, researchers can gain valuable insights into various disease conditions such as inflammatory disorders, respiratory diseases, and autoimmune disorders.
Overall, the Human Elastase-2B (ELA2B) ELISA Kit is a valuable tool for researchers looking to study the role of elastase-2B in disease pathology and develop potential therapies targeting this enzyme. Get accurate and reliable results with this advanced ELISA kit from Assay Genie.
Product Name:
Human Elastase 2B (ELA2B) ELISA Kit
Product Code:
HUDL00929
Size:
96 Assays
Target:
ELA2B
Synonyms:
CELA2B, Chymotrypsin-Like Elastase Family Member 2B, Pancreatic Elastase IIB
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
