The Human Elastase 3A (ELA3A) ELISA Kit is specifically designed for the precise and reliable detection of elastase 3A levels in human biological samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an ideal tool for a wide range of research applications.Elastase 3A is a key enzyme involved in various physiological processes, including inflammation, tissue remodeling, and immune response. Its dysregulation has been linked to numerous diseases, such as chronic obstructive pulmonary disease (COPD), cystic fibrosis, and inflammatory bowel disease, highlighting its importance as a potential biomarker for disease diagnosis and treatment monitoring.
Overall, the Human Elastase 3A (ELA3A) ELISA Kit provides researchers with a reliable and effective tool for studying the role of elastase 3A in health and disease, ultimately advancing our understanding of its implications in human physiology and pathology.
Product Name:
Human Elastase 3A (ELA3A) ELISA Kit
Product Code:
HUDL00931
Size:
96 Assays
Target:
ELA3A
Synonyms:
CELA3A, ELA3, Chymotrypsin-Like Elastase Family Member 3A, Protease E
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
