Human Ep-CAM/CD326 (Epithelial Cell Adhesion Molecule) CLIA Kit
The Human Ep-CAM (CD326) CLIA Kit is a powerful tool for the detection of Epithelial Cell Adhesion Molecule (Ep-CAM) levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit provides researchers with reliable and consistent results for a variety of research purposes.Ep-CAM is a critical cell adhesion molecule that plays a vital role in cell-to-cell interactions and cellular signaling pathways. Its overexpression is associated with various types of cancer, making it a valuable biomarker for cancer research and potential diagnostic and therapeutic applications.
By utilizing the Human Ep-CAM (CD326) CLIA Kit, researchers can accurately measure Ep-CAM levels in human samples, advancing our understanding of its role in cancer progression and other diseases. This kit is an essential tool for studying Ep-CAM biology and developing targeted treatments for diseases influenced by Ep-CAM expression.
Product Name:
Human Ep-CAM/CD326 (Epithelial Cell Adhesion Molecule) CLIA Kit
SKU:
HUES01039
Target:
Human Ep-CAM/CD326 (Epithelial Cell Adhesion Molecule)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 IU/mL
Detection range:
0.31-20 IU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Ep-CAM/CD326. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Ep-CAM/CD326 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Ep-CAM/CD326, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Ep-CAM/CD326. You can calculate the concentration of Human Ep-CAM/CD326 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
91-101
92-107
Average (%)
105
96
99
1:4
Range (%)
84-100
89-103
89-99
Average (%)
91
96
94
1:8
Range (%)
96-113
102-118
98-111
Average (%)
104
110
103
1:16
Range (%)
93-105
102-121
91-101
Average (%)
99
110
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
87-97
92
Cell culture media (n=5)
96-114
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.91
1.91
7.3
0.86
1.75
7.13
Standard deviation
0.09
0.22
0.75
0.07
0.14
0.49
C V (%)
9.89
11.52
10.27
8.14
8.0
6.87
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Ep-CAM/CD326 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Ep-CAM/CD326 in samples. No significant cross-reactivity or interference between Human Ep-CAM/CD326 and analogues was observed.
