The Human EphA1 (Ephrin Type-A Receptor 1) CLIA Kit is a cutting-edge assay designed for the accurate detection of EphA1 levels in human samples. With high sensitivity and specificity, this kit ensures reliable and reproducible results, making it ideal for a wide range of research applications.EphA1 is a key receptor involved in cell signaling and tissue patterning, playing a crucial role in various physiological and pathological processes. Dysregulation of EphA1 has been linked to cancer, autoimmune diseases, and neurological disorders, making it a valuable biomarker for understanding disease mechanisms and developing targeted therapies.
With the Human EphA1 CLIA Kit, researchers can uncover new insights into the role of EphA1 in health and disease, paving the way for advancements in personalized medicine and precision therapeutics. Partner with AssayGenie for reliable and innovative solutions in life science research.
Product Name:
Human EPHA1 (Ephrin Type A Receptor 1) CLIA Kit
SKU:
HUES00612
Target:
Human EPHA1 (Ephrin Type A Receptor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EPHA1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EPHA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EPHA1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EPHA1. You can calculate the concentration of Human EPHA1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
94-109
91-103
Average (%)
91
101
97
1:4
Range (%)
96-111
97-111
87-100
Average (%)
104
104
93
1:8
Range (%)
95-107
102-116
87-97
Average (%)
101
108
92
1:16
Range (%)
95-108
87-102
99-114
Average (%)
101
93
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
96-112
102
Cell culture media (n=5)
98-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
183.06
424.08
1717.2
189.39
390.42
1573.46
Standard deviation
19.57
34.6
122.44
17.94
31.94
165.37
C V (%)
10.69
8.16
7.13
9.47
8.18
10.51
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EPHA1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EPHA1 in samples. No significant cross-reactivity or interference between Human EPHA1 and analogues was observed.
