The Human EphA10 (Ephrin Type-A Receptor 10) CLIA Kit is a cutting-edge assay designed for the precise quantification of EphA10 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an invaluable tool for various research applications.EphA10 is a critical receptor involved in cell signaling and regulation, playing a key role in processes such as cell migration, adhesion, and differentiation.
Dysregulation of EphA10 has been implicated in various diseases including cancer, neurological disorders, and inflammatory conditions, highlighting its significance as a potential biomarker for disease detection and treatment development. Harness the power of the Human EphA10 CLIA Kit to advance your research and gain valuable insights into the role of EphA10 in health and disease.
Product Name:
Human EPHA10 (Ephrin Type A Receptor 10) CLIA Kit
SKU:
HUES00660
Target:
Human EPHA10 (Ephrin Type A Receptor 10)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EPHA10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EPHA10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EPHA10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EPHA10. You can calculate the concentration of Human EPHA10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
85-96
89-103
Average (%)
110
90
96
1:4
Range (%)
97-111
88-103
100-116
Average (%)
104
96
109
1:8
Range (%)
91-103
94-104
99-113
Average (%)
97
99
105
1:16
Range (%)
94-104
97-114
89-103
Average (%)
99
104
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
97-111
105
Cell culture media (n=5)
99-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
182.63
487.2
1547.13
180.25
457.45
1549.67
Standard deviation
19.29
47.31
165.54
16.96
41.9
165.19
C V (%)
10.56
9.71
10.7
9.41
9.16
10.66
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EPHA10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EPHA10 in samples. No significant cross-reactivity or interference between Human EPHA10 and analogues was observed.
