Human Epithelial Membrane Protein 3 (EMP3) ELISA Kit
The Human Epithelial Membrane Protein 3 (EMP3) ELISA Kit is a cutting-edge tool designed for the precise measurement of EMP3 levels in human samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for a variety of research applications.EMP3 is a vital protein that plays a key role in various biological processes, including cell growth, migration, and differentiation. Dysregulation of EMP3 has been implicated in numerous diseases, such as cancer, neurological disorders, and immune-related conditions, highlighting its significance as a potential biomarker for disease diagnosis and therapeutic development.
With the Human EMP3 ELISA Kit, researchers can delve deeper into the intricate mechanisms involving EMP3 and explore its implications in disease pathogenesis, ultimately paving the way for innovative treatments and interventions. Trust in this advanced kit to unlock new insights and drive transformative discoveries in the field of biomedical research.
Product Name:
Human Epithelial Membrane Protein 3 (EMP3) ELISA Kit
Product Code:
HUDL00941
Size:
96 Assays
Target:
EMP3
Synonyms:
YMP, HNMP-1, Hematopoietic neural membrane protein 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
