The Human EPO (Erythropoietin) CLIA Kit is a reliable and accurate tool for measuring erythropoietin levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring precise and consistent results for various research purposes.Erythropoietin is a critical hormone that regulates the production of red blood cells, playing a key role in maintaining oxygen transport in the body.
Imbalances in erythropoietin levels can lead to conditions such as anemia and kidney disease, making it a valuable biomarker for diagnosing and monitoring these disorders.With the Human EPO CLIA Kit, researchers can easily study erythropoietin levels in different biological samples, helping to advance our understanding of hematological disorders and potentially leading to the development of new therapeutic strategies.
Product Name:
Human EPO (Erythropoietin) CLIA Kit
SKU:
HUES00064
Target:
Human EPO (Erythropoietin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EPO. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EPO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EPO. You can calculate the concentration of Human EPO in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-116
93-110
98-114
Average (%)
109
100
104
1:4
Range (%)
98-115
87-100
86-98
Average (%)
106
93
91
1:8
Range (%)
97-111
100-116
92-106
Average (%)
103
109
99
1:16
Range (%)
94-105
103-121
88-102
Average (%)
99
110
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
94-107
102
Cell culture media (n=5)
95-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.84
104.28
326.11
39.08
94.62
296.04
Standard deviation
4.14
7.36
32.71
3.56
11.07
22.11
C V (%)
10.14
7.06
10.03
9.11
11.7
7.47
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EPO concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EPO in samples. No significant cross-reactivity or interference between Human EPO and analogues was observed.
